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                    Products > Loading Buffer > Gel Loading Dye, Three-colour (6X)(M9061)

                    Gel Loading Dye, Three-colour (6X)(M9061)

                    Contents
                    Gel Loading Dye, Three-colour (6X):

                    10 mM Tris-HCL(pH 7.6)
                    0.03% Bromophenol Blue
                    0.03% Xylene Cyanol
                    0.15% Orange G
                    60% Glycerol
                    60 mM EDTA
                     
                    Description
                    Gel Loading Dye, Three-colour (6X) is a pre-mixed Loading Buffer with three tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue, xylene cyanol and orange G are the standard tracking dye for electrophoresis.

                    Storage 
                    Store at RT
                    Quality Assurance Statement
                    Gel Loading Dye, Three-colour (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
                     
                    Dye Migrate Rate (on a standard 1% TAE or TBE agarose gel) 
                    Xylene Cyanol
                    TAE: 4160 bp
                    TBE: 3030 bp

                    Bromophenol Blue 
                    TAE: 370 bp
                    TBE: 220 bp

                    Orange G
                    TAE/TBE<50 bp

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