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                    Products > Loading Buffer > Gel Loading Dye, SDS+(6X)(M9081)

                    Gel Loading Dye, SDS+(6X)(M9081)

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                    Contents
                    Gel Loading Dye, SDS+ (6X):

                    10 mM Tris-HCL(pH 7.6)
                    0.03% Bromophenol Blue
                    0.03% Xylene Cyanol
                    60% Glycerol
                    1% SDS
                    100 mM EDTA
                     
                    Description
                    Gel Loading Dye, SDS +(6X) is a pre-mixed Loading Buffer with two tracking dye for agarose and non-denaturing poylacrylamide gel electrophoresis.This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue and xylene cyanol are the standard tracking dye for electrophoresis.

                    Storage 
                    Store at RT

                     

                    Quality Assurance Statement
                    Gel Loading Dye, SDS+ (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
                     
                    Dye Migrate Rate (on a standard 1% TAE or TBE agarose gel) 
                    Xylene Cyanol
                    TAE: 4160 bp
                    TBE: 3030 bp

                    Bromophenol Blue 
                    TAE: 370 bp
                    TBE: 220 bp


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