Gel Loading Dye (6X):
10 mM Tris-HCL（pH 7.6）
0.15% Orange G
0.03% Xylene Cyanol
60 mM EDTA
Gel Loading Dye, Orange (6X) is a pre-mixed Loading Buffer with two tracking dye for agarose
and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium
(up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Xylene cyanol and Orange G are the standard tracking dye for electrophoresis.
Store at RT
Quality Assurance Statement
Gel Loading Dye, Orange (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
Dye Migrate Rate (on a standard 1% TAE or TBE agarose gel)
TAE: 4160 bp
TBE: 3030 bp