? FSTM Taq DNA Polymerase has two concentrations with 2.5 U/μl and 5 U/μl. Default package is 5
? 10×FSTM PCR Buffer (Mg2+ Plus) can replace with 10×PCR Buffer (Mg2+ free) and 25 mM MgCl2.
DNA polymerase. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does, can be used in a variety of PCR. The FSTM PCR Buffer , designed for FSTMTaq DNA polymerase, can be used in fast amplification reaction.The elongation rate of FSTM Taq DNA polymerase is 2-fold higher than the one of regular Taq DNA polymerase, which shortens the amplification time by half .
Fast rate of elongation: elongation velocity can reach to 3 kb/min, 2x higher than regular Taq DNA polymerase
Highly thermostable : have a half-life of over 40 min at 95°C incubation Generates 3'-dA overhangs PCR products
Generate PCR product for TA cloning
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests
Functionally tested in amplification of a single-copy gene from human genomic DNA
20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 %TW 20, 0.5 % NP 40, 50 % Glycerol
10X FSTM PCR Buffer
200 mM Tris-Cl(PH 8.8), 100 mM KCl, 100mM (NH4)2SO4, 16 mM MgSO4, 1% Triton-X-100.
Store all components at –20°C
Protocol for PCR
All solutions should be thawed on ice, gently vortexed and briefly centrifuged.
Add in a thin walled PCR tube on ice:
For a total 50μl reaction volume
·Gently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.
·Overlay the sample with mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid.
·Place samples in a thermocycler and start the program.
PCR Cycling Protocol