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                    Products > Fast PCR > FS Taq DNA Polymerase (P1071/P1072/P1073/P1074)

                    FS Taq DNA Polymerase (P1071/P1072/P1073/P1074)

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                    Contents
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                    Note 
                    ? FSTM Taq DNA Polymerase has two concentrations with 2.5 U/μl and 5 U/μl. Default package is 5

                    U/μl.
                    ? 10×FSTM PCR Buffer (Mg2+ Plus) can replace with 10×PCR Buffer (Mg2+ free) and 25 mM MgCl2.

                     

                    Please choose the appropriate package for your experiment.

                    Description
                    FSTM Taq DNA polymerase, developed by Dongsheng Co.,LTD. is the latest generation Taq-based

                    DNA polymerase. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does, can be used in a variety of PCR. The FSTM PCR Buffer , designed for FSTMTaq DNA polymerase, can be used in fast amplification reaction.The elongation rate of FSTM Taq DNA polymerase is 2-fold higher than the one of regular Taq DNA polymerase, which shortens the amplification time by half .

                    Features
                    Fast rate of elongation: elongation velocity can reach to 3 kb/min, 2x higher than regular Taq DNA polymerase
                    Highly thermostable : have a half-life of over 40 min at  95°C incubation Generates 3'-dA overhangs PCR products

                    Applications
                    Routine PCR
                    PCR labeling
                    PCR sequencing
                    Generate PCR product for TA cloning

                    Unit Definition
                    One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

                    Quality Control 
                    The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests

                    Functionally tested in amplification of a single-copy gene from human genomic DNA

                    Storage Buffer
                    20 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5 %TW 20, 0.5 % NP 40, 50 % Glycerol

                    10X FSTM PCR Buffer

                    200 mM Tris-Cl(PH 8.8), 100 mM KCl, 100mM (NH4)2SO4, 16 mM MgSO4, 1% Triton-X-100.

                    Store all components at –20°C

                    Protocol for PCR
                    All solutions should be thawed on ice, gently vortexed and briefly centrifuged.
                    Add in a thin walled PCR tube on ice:


                    For a total 50μl reaction volume


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                    ·Gently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.
                    ·Overlay the sample with mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid.
                    ·Place samples in a thermocycler and start the program.


                    PCR Cycling Protocol


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