FSTM Kit contains a FSTM Taq DNA Polymerase and a 2X premixed, ready-to-use FSTM reaction Mix. The FSTM reaction mix is supplied as a 2X concentrated solution, which contains dNTPs and all other PCR components, except DNA template and primers. To prepare the final PCR reaction system, mix FSTM Taq DNA Polymerase and the FSTM reaction mix, then add primer and template. The reaction Mix has higher amplification sensitivity due to optimized components and enhancer.
FSTM Taq DNA polymerase, developed by Dongsheng Co., LTD, is the latest generation Taq-
based DNA polymerase. It possesses high amplification efficiency as Taq polymerase does, and fast elongation ability as KOD polymerase does, and can be use in various kinds of PCR. The FSTM PCR Buffer is designed for FSTMTaq DNA polymerase, and can be used in fast amplification reaction. FSTM Taq DNA polymerase has an elongation rate 2x higher than regular Taq DNA polymerase, and can shorten the amplification time by half. It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity, which results in a 3'-dA overhangs PCR product.
Convenient: Dongseng recombinant Taq DNA Polymerase in a ready-to-use mix
Fast: saves time due to reduced number of pipetting steps
Reproducible: lower contamination and pipetting error risk
Flexible: the amount of DNA polymerase used is flexible
High throughput PCR for long and complex template
High reproducible PCR for long and complex template
Generation of PCR products for TA cloning
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests. Functionally tested in amplification of a single-copy gene from human genomic DNA.
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Store all components at -20°C
Repeated freeze-thaw cycles do not reduce the activityof the reactions
Protocol for PCR
All solutions should be thawed on ice, gently vortexed and briefly centrifuged.
Add in a thin walled PCR tube on ice:
For a total 50μl reaction volume
Gently vortex the sample and briefly centrifuge to collect all drops to the bottom of the tube.
·Overlay the sample with mineral oil or add an appropriate amount of wax. This step may be omitted if the thermal cycler is equipped with a heated lid.
·Place samples in a thermocycler and start the program.
PCR Cycling Protocol