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                    Products > High Fidelity PCR > Pfu DNA Polymerase (P1021/P1022/P1023/P1024)

                    Pfu DNA Polymerase (P1021/P1022/P1023/P1024)

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                    Contents
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                    Note

                    10×PCR Buffer (Mg2+ Plus) can replace with 10×PCR Buffer (Mg2+ free) and 25 mM MgCl2. Please choose the appropriate package for your experiment.

                    Description
                    Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase.  Unlike Taq DNA polymerase, highly thermostable Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis. 

                    Features
                    3'-5' exonuclease activity provides a low error rate
                    One of the most thermostable DNA polymerases known
                    Lack of extendase activity means no unwanted 3’ overhangs
                    Optimal for blunt-ended PCR cloning
                    Optimum temperature near 75°C
                    95% active after 1hour incubation at 98°C
                     
                    Applications
                    High-fidelity PCR and primer-extension reactions
                    PCR cloning and blunt-ended amplification product generation

                    Unit Definition
                    One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nM of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

                    Storage Buffer 

                    20mM Tris-HCl ( pH8.0), 100mM KCl, 3mM MgCl2 1mM DTT,0.1% NP-40 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol


                    10X Pfu Buffer

                    200mM Tris-HCl PH8.8,100mM KCl,100mM (NH4)2SO4,20mM MgSO4,1% Tritonx-100,1mg/ml BSA


                    Store all components at –20°C 


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