Gel Loading Dye, Blue (6X):
10 mM Tris-HCL（pH 7.6）
0.03% Bromophenol Blue
60 mM EDTA
Gel Loading Dye, Blue (6X) is a pre-mixed Loading Buffer with one tracking dye for agarose
and non-denaturing poylacrylamide gel electrophoresis. EDTA is included to chelate magnesium
(up to 10 mM) in enzymatic reactions, thereby stopping the reaction. Bromophenol blue is the
standard tracking dye for electrophoresis.
Store at RT
Quality Assurance Statement
Gel Loading Dye, Blue (6X) is assayed for contaminating endonuclease, exonuclease and RNase activity.
Dye migrate rate
Bromophenol Blue migrates at approximately 370 bp on a standard 1% TAE agarose gel, and 220 bp on a standard 1% TBE agarose gel.