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                    Products > DNA Purification Accessary Reagents > LE-Agarose 1200(N9051)

                    LE-Agarose 1200(N9051)

                    LE-Agarose 1200Catalog #SizePrice
                    N9051500g

                     

                    Description

                    LE-Agarose 1200 is a low EEO, high gel strength agarose for analysis of nucleic acid molecules. Typical applications include electrophoretic separation of DNA and RNA, Southern & Northern blotting, and also lmmunodiffuson and Ouchterlony of proteins.

                    Analytical Specifications
                    EEO (-m r)0.1-0.15
                    Water Content≤10%
                    Sulfate (SO4)0.15-0.2%
                    Gel Strength (1% gel)≥1200 g/cm2
                    Gelling Point (1.5% gel)33±1.5oC
                    Melting Point (1.5% gel)87±1.5oC

                    Concentrations for DNA separation
                    The appropriate agarose concentration depends on the size of the DNA fragments to be separated, the buffer used, and the applied voltage. With IX TAE buffer, the suggested concentrations for DNA sizes in base pairs (bp)  follows :

                    size range (bp)cincentration
                    800-100000.8%
                    400-80001.0%
                    200-40001.5%
                    100-30002.0%

                     


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