<address id="rnhrh"><form id="rnhrh"></form></address>

      <form id="rnhrh"></form>

                    <form id="rnhrh"></form>

                    Products > DNA Purification Accessary Reagents > DNase I (Lyophilized Powder)(N9066)

                    DNase I (Lyophilized Powder)(N9066)

                    DNase I

                    Catalog #




                    Cat No. N9066, 1g


                    Specific Activity: ≥ 2000 U/mg 

                    Form: Contains 1g DNase I, Lyophilized Powder


                    Store at -20°C for 2 years.


                    DNase I is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA: DNA hybrids.



                    ? Extracted from the bovine pancreas.


                    ? Degradation of DNA template in transcription reactions

                    ? Removal of contaminating genomic DNA from RNA samples

                    ? DNase I footprinting

                    ? Nick translation


                    Definition of Activity Unit

                    One unit of the enzyme completely degrades 1 μg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 μg of pUC19 DNA.

                    Bovine pancreas.

                    Molecular Weight
                    38 kDa monomer.


                    Quality Control

                    Functionally tested for digestion of template DNA after in vitro transcription.


                    Inhibition and Inactivation
                    Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM.

                    Inactivated by heating at 65°C for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+).


                    DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

                    正规手机购彩 <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>