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                    Products > DNA Purification Accessary Reagents > DNase I (Lyophilized Powder)(N9066)

                    DNase I (Lyophilized Powder)(N9066)

                    DNase I

                    Catalog #

                    Size

                    N9066

                    1g



                    Cat No. N9066, 1g

                     

                    Specific Activity: ≥ 2000 U/mg 

                    Form: Contains 1g DNase I, Lyophilized Powder

                     

                    Store at -20°C for 2 years.

                     

                    Description
                    DNase I is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5′-phosphorylated and 3′-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA: DNA hybrids.

                     

                    Features

                    ? Extracted from the bovine pancreas.


                    Applications

                    ? Degradation of DNA template in transcription reactions

                    ? Removal of contaminating genomic DNA from RNA samples

                    ? DNase I footprinting

                    ? Nick translation

                     

                    Definition of Activity Unit

                    One unit of the enzyme completely degrades 1 μg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 μg of pUC19 DNA.


                    Source
                    Bovine pancreas.


                    Molecular Weight
                    38 kDa monomer.

                     

                    Quality Control

                    Functionally tested for digestion of template DNA after in vitro transcription.

                     

                    Inhibition and Inactivation
                    Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM.

                    Inactivated by heating at 65°C for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+).


                    Note

                    DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.


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