RNase (10 mg/ml)
Wash Buffer PS
Wash Buffer PE
30 ml × 2
Elution Buffer TE (10mM Tris-HCl, 1mM EDTA, pH 8.5)
The Quick Plant Genomic DNA extraction system uses the silica-based membrane technology for simple and fast isolation of genomic DNA (gDNA). The buffer system is optimized to allow selective binding of DNA to the silica-based membrane. The simple centrifugation procedure can completely removes impurities such as proteins, lipids and polysaccharides. Pure DNA is then eluted in water or low-salt buffer, ready to use.
The kit is suitable for high quality genomic DNA purification of tissue from a wide variety of plant species, such as arabidopsis, tobacco and rice, etc.
Purified DNA is free of impurities and enzyme inhibitors, and have an A260/280=1.7-1.9, is suitable for applications such as:
? Restriction enzyme digestion
? Southern blotting
? Fast - procedure takes less than 60 min
? High efficiency - 2-20 μg of genomic DNA from less than 100 mg tissue
? Universal - purifies genomic DNA from various sources of plant species
? Convenient - purified DNA can be directly used in all molecular biology applications
? High purity - purified DNA without enzyme inhibitors, RNA or proteins, ready for downstream applications
Store at room temperature for up to 1 year. For longer storage, RNase could be stored at 4 ?C or -20 ?C.