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                    Products > Virus-related Products > QPCR Hotstart Taq DNA Polymerase real-time pcr P1101 P1102 P1103 P1104

                    QPCR Hotstart Taq DNA Polymerase real-time pcr P1101 P1102 P1103 P1104

                    QPCR Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase modified by antibody-like technology, and the activity is wholly inhibited at room temperature, which results in less non-specific amplifications. It is a special polymerase for real-time quantitative PCR (qPCR), both dye method and probe method.

                    QPCR Hotstart Taq DNA Polymerase

                    For research use only

                    Components  

                    Component

                    P1101 

                    250 U

                    P1102 

                    1,000 U

                    P1103 

                    3,000 U

                    P1104 

                    18,000 U

                    qPCR Hotstart Taq DNA Polymerase (5 U/μl)

                    50 μl

                    200 μl

                    200 μl × 3

                    100 μl × 36

                    10× qPCR Buffer[1] 

                    1.25 ml

                    1.25 ml × 2

                    1.25 ml × 6

                    1.25 ml × 36

                    [1] qPCR Buffer contains Mg2+, dNTPs and other components that are necessary for qPCR.

                     

                    Storage

                    This reagent should be kept at -20°C. The shelf life is 2 years when stored properly.

                     

                    Description

                    QPCR Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase modified by antibody-like technology, and the activity is wholly inhibited at room temperature, which results in less non-specific amplifications. Depending on high temperature to activate the enzyme activity, the reaction has very high specificity and sensitivity. QPCR Hotstart Taq DNA Polymerase adopts advanced production technology, does not introduce contamination of animal-derived DNA. The enzyme has stronger stability, and incomparable advantages over antibody-modified hot-start Taq DNA polymerase. The pre-denaturation time is reduced to 3 minutes, and the amplification efficiency is higher than that of most chemical-modified hot-start DNA polymerase. It is a special polymerase for real-time quantitative PCR (qPCR), both dye method and probe method.

                     

                    Unit Definition

                    Using activated salmon sperm DNA as template/primer, the activity of ingesting 10 nmol total nucleotides as acid insoluble substances within 30 min at 74°C is defined as 1 unit of activity (U).

                     

                    Applications

                    High-specificity amplification & multiplex qPCR: hot-start polymerase with antibody-like modification

                    Thermostable: half-life over 40 min at 95°C incubation

                    Generates 3'-dA overhangs PCR products


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