QPCR Hotstart Taq DNA Polymerase
For research use only
qPCR Hotstart Taq DNA Polymerase (5 U/μl)
200 μl × 3
100 μl × 36
10× qPCR Buffer
1.25 ml × 2
1.25 ml × 6
1.25 ml × 36
 qPCR Buffer contains Mg2+, dNTPs and other components that are necessary for qPCR.
This reagent should be kept at -20°C. The shelf life is 2 years when stored properly.
QPCR Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase modified by antibody-like technology, and the activity is wholly inhibited at room temperature, which results in less non-specific amplifications. Depending on high temperature to activate the enzyme activity, the reaction has very high specificity and sensitivity. QPCR Hotstart Taq DNA Polymerase adopts advanced production technology, does not introduce contamination of animal-derived DNA. The enzyme has stronger stability, and incomparable advantages over antibody-modified hot-start Taq DNA polymerase. The pre-denaturation time is reduced to 3 minutes, and the amplification efficiency is higher than that of most chemical-modified hot-start DNA polymerase. It is a special polymerase for real-time quantitative PCR (qPCR), both dye method and probe method.
Using activated salmon sperm DNA as template/primer, the activity of ingesting 10 nmol total nucleotides as acid insoluble substances within 30 min at 74°C is defined as 1 unit of activity (U).
High-specificity amplification & multiplex qPCR: hot-start polymerase with antibody-like modification
Thermostable: half-life over 40 min at 95°C incubation
Generates 3'-dA overhangs PCR products