<address id="rnhrh"><form id="rnhrh"></form></address>

      <form id="rnhrh"></form>

                    <form id="rnhrh"></form>

                    Products > Virus-related Products > 2019-nCoV-abEN Pseudovirus V1001/V1002/V1003

                    2019-nCoV-abEN Pseudovirus V1001/V1002/V1003

                     

                    2019-nCoV-abEN Pseudovirus

                    Catalog No.: V1001  Size: 1 ml

                    Catalog No.: V1002  Size: 1 ml * 5

                    Catalog No.: V1003  Size: 1 ml * 10

                     

                    Description

                    Part of the ORF1a/b gene sequence, Gene E and Gene N coding region sequence of 2019-nCoV were obtained by chemical synthesis, and cloned into a retrovirus vector. The pseudovirus was prepared in 293T cells, and then concentrated and purified by ultra-fast centrifugation. The pseudovirus is composed of retrovirus envelope and above sequences. It can be used as a positive control for viral RNA extraction experiment and qPCR test.

                     

                    Product component structure

                     

                     

                    Product sequence information

                    See the attachment.

                     

                    Main ingredients

                    Glucose, potassium dihydrogen phosphate, disodium phosphate, sodium chloride, potassium chloride and 2019-nCoV-abEN pseudoviruses.

                     

                    Storage conditions and validity

                    Frozen below -20°C, valid for 6 months.

                     

                    Usage

                    1. Melting of pseudovirus: remove the pseudovirus from the refrigerator at -20°C, melt it in an ice bath or put it in a 4 °C environment, and then conduct relevant experimental operations after it has completely melted;

                    2. Inactivation (optional) of pseudovirus: absorb the required amount of pseudovirus in the biological safety cabin and inactivate it in the EP tube at 56°C for 30min;

                    3. Nucleic acid extraction and qPCR detection of pseudovirus: carry out relevant experimental operations according to the instructions of nucleic acid extraction kit and qPCR detection kit.

                     

                    Precautions

                    1. Freezing-thawing will reduce the stability of the pseudovirus, thus affecting the effect of nucleic acid extraction and qPCR test results. Repeated freezing-thawing should be avoided;

                    2. If the product needs to be diluted, phosphate buffer (PBS) or normal saline (0.9% NaCl) can be used;

                    3. In case of accidental splash to eyes, skin or other body parts, please immediately rinse with plenty of water;

                    4. The experimental wastes generated by the use of this product shall be treated according to the requirements of medical waste treatment after high-pressure sterilization.


                    The attachment
                    :


                    1. ORF1 a/b sequence

                    ATCGTGTTGTCTGTACTGCCGTTGCCACATAGATCATCCA

                    AATCCTAAAGGATTTTGTGACTTAAAAGGTAAGTATGTAC

                    AAATACCTACAACTTGTGCTAATGACCCTGTGGGTTTTAC

                    ACTTAAAAACACAGTCTGTACCGTCTGCGGTATGTGGAAA

                    GGTTATGGCTGTAGTTGTGATCAACTCCGCGAACCCATGC

                    TTCAGTCAGCTGATGCACAATCGTTTTTAAACGGGTTTGC

                    GGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCA

                    CTAGTACTGATGTCGTATACAGGGCTTTTGACATCTACAA

                    TGATAAAGTAGCTGGTTTTGCTAAATTCCTAAAAACTAAT

                    TGTTGTCGCTTCCAAGAAAAGGACGAAGATGACAATTTAA

                    TTGATTCTTACTTTGTAGTTAAGAGACACACTTTCTCTAA

                    CTACCAACATGAAGAAACAATTTATAATTTACTTAAGGAT

                    TGTCCAGCTGTTGCTAAACAT

                    2. E Gene sequence

                    ATGTACTCATTCGTTTCGGAAGAGACAGGTACGTTAATAG

                    TTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTT

                    GCTAGTTACACTAGCCATCCTTACTGCGCTTCGATTGTGT

                    GCGTACTGCTGCAATATTGTTAACGTGAGTCTTGTAAAAC

                    CTTCTTTTTACGTTTACTCTCGTGTTAAAAATCTGAATTC

                    TTCTAGAGTTCCTGATCTTCTGGTCTAA

                    3.N Gene sequence

                    ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCC

                    GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAA

                    CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGT

                    CGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCA

                    CCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCC

                    TCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCA

                    GATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAA

                    TTCGTGGTGGTGACGGTAAAATGAAAGATCTCAGTCCAAG

                    ATGGTATTTCTACTACCTAGGAACTGGGCCAGAAGCTGGA

                    CTTCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTG

                    CAACTGAGGGAGCCTTGAATACACCAAAAGATCACATTGG

                    CACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAA

                    CTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAG

                    AAGGGAGCAGAGGCGGCAGTCAAGCCTCTTCTCGTTCCTC

                    ATCACGTAGTCGCAACAGTTCAAGAAATTCAACTCCAGGC

                    AGCAGTAGGGGAACTTCTCCTGCTAGAATGGCTGGCAATG

                    GCGGTGATGCTGCTCTTGCTTTGCTGCTGCTTGACAGATT

                    GAACCAGCTTGAGAGCAAAATGTCTGGTAAAGGCCAACAA

                    CAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGG

                    CTTCTAAGAAGCCTCGGCAAAAACGTACTGCCACTAAAGC

                    ATACAATGTAACACAAGCTTTCGGCAGACGTGGTCCAGAA

                    CAAACCCAAGGAAATTTTGGGGACCAGGAACTAATCAGAC

                    AAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATT

                    TGCCCCCAGCGCTTCAGCGTTCTTCGGAATGTCGCGCATT

                    GGCATGGAAGTCACACCTTCGGGAACGTGGTTGACCTACA

                    CAGGTGCCATCAAATTGGATGACAAAGATCCAAATTTCAA

                    AGATCAAGTCATTTTGCTGAATAAGCATATTGACGCATAC

                    AAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGA

                    AGAAGGCTGATGAAACTCAAGCCTTACCGCAGAGACAGAA

                    GAAACAGCAAACTGTGACTCTTCTTCCTGCTGCAGATTTG

                    GATGATTTCTCCAAACAATTGCAACAATCCATGAGCAGTG

                    CTGACTCAACTCAGGCCTAA

                    (over)

                     

                     

                     

                    正规手机购彩 <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>