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                    Marker12

                    Quick Details

                    • CAS No.: 100403-24-5

                    • EINECS No.: 231-791-2

                    • Place of Origin: Guangdong, China (Mainland)

                    • Purity: 75ng/ul

                    • Classification: High Purity Reagents

                    • Brand Name: DSB

                    • Model Number: M1141/M1142

                    • Color: Blue

                    Packaging & Delivery

                    Packaging DetailsItem No: M1141 
                    Size: 50ug 
                    Item No: M1142 
                    Size: 5x50ug 
                    Pack in vials
                    Delivery Time1-2 weeks

                    Concentration: 75ng/μl         

                     

                    Recommended Loading

                    2-5 μl/Lane.

                     

                    Concentration

                    Typical Bands     125 ng/5μl

                    Other Bands      50 ng/5μl

                     

                    Recommended Electrophoresis Condition

                    5 μl/Lane, 8 cm 1.7 %Agarose Gel, 0.5×TBE, 5V/cm, 1h.

                     

                    Contents(bp)

                    200 ,400,750,1,000,1,500,2,000

                     

                    Storage

                    1 year at room temperature, for long-time preservation, please storage at -20°C .

                     

                    Description

                    Marker 12 is ideal for determining the size of double-stranded DNA from 200 to 2,000 base pairs. The ladder consists of 6 linear double-stranded fragments .The 1,000bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 1,000bp are 50ng. The 1,000bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.  

                     

                    Recommendations

                    Do not heat before loading.

                    For quantification, adjust the concentration of the sample to equalize

                    it approximately with the amount of DNA in the nearest band of the ladder.

                    Visualize DNA by staining with ethidium bromide or with SYBR? Green I.

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